SPECIFICATION
- The system automated for both real-time PCR and post-PCR (end-point) analysis using an in-built Peltier-based PCR machine.
- System support applications including absolute quantitation, simultaneous analysis data for relative quantitation of Unlimited plates of 96 wells each, (4-6 colour multiplexing), allelic discrimination (SNP), dissociation curve analysis, as well as pathogen detection and plus minus assay using internal positive control.
- The instrument would have a 96-well sample block of 0.1ml capacity, able to run fast and standard runs on the same block. It can also have 6 separate Peltier-controlled blocks with a fixed gradient.
- The system complete the Fast 40 cycle protocol in less than 40 minutes and the standard protocol in under 2 hours.
- The system have an LCD touch display with a USB interface to export the data.
- The temperature range of the thermos block 4°C to 99°C with an accuracy of ±0.25°C
- The system have a complete solution for a Fast real-time PCR machine: o Fast instruments, Fast reagents, Fast protocols, and Fast assays.
- Sample Ramp Rate: fast Mode: ±3.5°C/sec
- Standard Mode: 1.6°C/sec
- Pear Block Ramp Rate: 5.0°C/sec, Temperature Uniformity: ±0.50°C, 30 seconds after clock start.
- The Excitation source a single blue LED light source, or a Tungsten Halogen or high-intensity Xenon lamp, and emission detection by photodiodes or a cooled CCD camera. There enough excitation and emission filters to cover the majority of dyes.
- The system flexible to support 96-well plates, individual tubes. and 8-strip tubes. The system quoted with interchangeable blocks.
- System software provide simultaneous analysis data for the relative quantitation of Unlimited plates of 96 wells each.
- The system have normalisation of the reaction due to non-PCR-related fluctuations and variations, which possible by using ROX™ or any other calibrated dye.
- The system support a reaction volume of 10-50 μ.
- The instrument software must be capable of detecting and analysing a different gene, SNP, or pathogen target in every well of the 96-well plate.
- The system have an easy door design for loading and unloading 96-well plates or individual 0.2 ml PCR tubes.
- The system collect data for all filters for all wells, regardless of plate setup. The software allow reanalysis of data so that data is never lost.
- The instrument pre-calibrated for at least seven dyes, including the following during installation at the customer site: FAMTM/SYBR Green 1, VICE/JOEM, NEDIM/TAMRAT™/ and ROXT™, The user able to use any of these dyes in an experiment without needing to recalibrate the instrument. The addition of new dyes possible without a hardware change.
- The system supplied with licensed full version software for primer and probe design, with comprehensive assay design and development guidelines for quantitative and qualitative real-time assays, to enable the designing of custom oligo assays.
- The system standardized for at least two homogeneous reaction chemistries, including SYBR Green I and dual colour TaqMan or four colour hybridization probes (FRET).
- The system offered pre-validated and functionally tested Gene Expression Assays, as well as SNP Genotyping Assays, and the flexibility to design specific assays for new templates of interest.
- The instrument software utilize a multi-component algorithm . designed to provide precise deconvolution of multiple dye signals to enable the simultaneous detection of multiple fluorophores,
- The system supplied with all the accessories required to function
- PC/Laptop - Branded i5 PC with Windows 10/11 Professional with LaserJet printer
- Online UPS - 2 KVA Online UPS (single Phase) with 2 hr backup must be provided
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