Flow Injection Analysis

Order Code: 32379

Category: Thermodynamics Lab

Features Professional analyzer for TH 165 Continuous  photometric  determination  of  the glucose concentration Sci-tech Flow Injection Analysis Apparatus Model 32379 supplements 32378. It uses the photometer in 32378 as a de...



SPECIFICATION

Features
Professional analyzer for TH 165
Continuous  photometric  determination  of  the glucose concentration

Sci-tech Flow Injection Analysis Apparatus Model 32379 supplements 32378. It uses the photometer in 32378 as a detector to detect the reaction product glucose.
The multi-channel pump permanently conveys three liquid flows into the FIA. The dissolved reaction products from TH 165 and an indicator reagent are first mixed in one chamber. The mixture then flows through a helical reaction loop. The conduction of the flow in the reaction loop enables an even distribution of all substances. Another indicator reagent is added in a second mixing chamber. After flowing through another reaction loop, the mixture enters the flow cell. There the light intensity is continuously measured with the photometer to determine the glucose concentration. To trigger the discoloration for the photometric measurement, a defined amount of the enzyme ‘glucose oxidase’ is injected through an injection valve. The indicator reagents and the enzyme ‘glucose oxidase’ are not included in the scope of delivery.
32379  enables  more  measurements  during  the experiment than a manual analysis. In addition, the reproducibility  is  improved  and  it  is  no  longer necessary to mix each individual sample.

Specifications
Continuous, photometric  determination of the glucose concentration in the product from 32378
PTFE flow cell for determining the concentration with the photometer from 32378
Multi-channel peristaltic pump for conveying the
product from 32378 and the indicator reagents
Injection valve, injection syringe and injection loop for adding the enzyme ‘glucose oxidase’ required for verification
2 mixing chambers for mixing the product and indicator reagents
2 PTFE reaction loops
3 DURAN glass beakers for indicator reagents and enzyme ‘glucose oxidase’
1 tank for waste

Technical Specifications
Flow cell travel length: 1cm
Multi-channel peristaltic pump
4 channels
max. flow rate per channel:  11mL/min  at 100min-1 and hose Di=1,42mm
Injection valve
6 connections
2 switch positions
Loops
Reaction loops: 1x 2000mm, 1x 4000mm
Injection loop: 1x 100mm
Tanks
Indicator reagents: 2x 250mL
Enzyme ‘glucose oxidase’: 1x 25mL
Waste: 1x 1000mL
Injection syringe: 1x 10mL

Experiments
Using the flow injection analysis (FIA)
Determining the concentration
Determining the yield for TH 165

Requirements
Mains Power 220 – 240V @ 50Hz, 1Ph

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